Bone fractures at the end of lay are a significant problem in egg-laying strainsof hens. The objective of the current study was to identify quantitative traitloci (QTL) associated with bone mineralization and strength in a chickenresource population. Layer (White Leghorn hens) and broiler (Cobb-Cobb roosters)lines were crossed to generate an F2 population of 508 hens over seven hatches,and 26 traits related to bone integrity, including bone mineral density (BMD)and content (BMC), were measured. Genotypes of 120 microsatellite markers on 28autosomal groups were determined, and interval mapping was conducted to identifyQTL regions. Twenty-three tests representing three chromosomal regions(chromosomes 4, 10 and 27) contained significant QTL that surpassed the 5%genome-wise threshold, and 47 tests representing 15 chromosomes identifiedsuggestive QTL that surpassed the 5% chromosome-wise threshold. Although nosignificant QTL influencing BMD and BMC were detected after adjusting forvariation in body weight and egg production, multiple suggestive QTL were found.These results support previous experiments demonstrating an important geneticregulation of bone strength in chickens, but suggest the regulation may be dueto the effects of multiple genes that each account for relatively small amountsof variation in bone strength.