Reduction in Salmonella enteritidis (SE) contamination is of importance forpoultry production as well as for food safety. The objectives of this study wereto identify potential genetic markers of antibody response to SE vaccine inyoung broiler chicks and then to confirm this linkage in broiler-crossoffspring, as well as to explore interactions of marker alleles with dam lineand sex. The initial identification of suggestive quantitative trait loci (QTL)markers for antibody response to SE vaccine was conducted by using bulkedsegregant analysis (BSA) with 58 microsatellite markers in a broiler breedermale line. Four unlinked microsatellites that had allele frequency differencesbetween the high and low antibody response DNA pools were selected forsubsequent analysis in a linkage study. Antibody response was measured in an F1population (n = 379) that was derived by crossing each of four males of thebroiler line with several dams from four genetically distant, highly inbredlines (Spanish, Fayoumi, and MHC-congenic G-B1 and G-B2 Leghorn). These crossesenabled us to evaluate the broiler sire QTL-marker allele effects and to exploreQTL interactions with the dam lines by individual genotyping. Each of the fourmicrosatellites identified by BSA in the broiler population had a significant (P< 0.05) association with F1 population antibody response in one or more sirefamilies. The effect of the interaction of microsatellite allele with dam lineor sex on antibody response was frequently significant. Microsatellite markerslinked to antibody response QTL were identified, and genetic interactions withdam line and sex were detected.