Pig Genome Update No. 14

September 1, 1995

  1. The Recent 9th North American Colloquium on Domestic Animal Cytogenetics and Gene Mapping (Texas)
  2. The American Society of Animal Science Meetings
  3. Ideas from Pig Gene Mappers Are Needed
  4. Information on Inherited Disorders in Pigs
  5. The Coming NRSP-8 Meetings and Pig Gene Mapping NC-210 Meeting

Cytogenetics and gene mapping were discussed at the recent 9th North American Colloquium on Domestic Animal Cytogenetics and Gene Mapping at College Station, Texas. Nearly 70 participants from 14 countries attended the three-day program.

The meeting also featured several speakers as part of a NAGRP sponsored "Symposium on Animal Gene Mapping." The first invited speaker, Dr. Mike Siciliano, M.D. Anderson Hospital and Tumor Institute, presented an interesting report, "Painting probes from hybrid cells for mapping and chromosome rearrangement identification." He reported his efforts with monochromosomal (human) somatic cell lines and their use in developing region specific probes.

The second invited speaker was Dr. Ruedi Fries from the Animal Production Institute in Munich. Dr. Fries' paper, "Comparative genome maps and positional candidate cloning in domestic species," was an extremely interesting presentation that examined the progress made using ZOO-Fish for comparative gene mapping and the ever-increasing role that positional cloning is expected to play in human and domestic animal gene identification.

The third invited speaker, Dr. Deryl Troyer, Department of Veterinary Anatomy and Physiology, Kansas State University, discussed the "Use of DISC-PCR in conjunction with other physical mapping methods." His excellent presentation discussed several techniques for physical mapping including FISH and DISC-PCR. He also outlined possible pitfalls of the procedure and presented results related to several pig chromosomes including chromosomes 1 and 7 in which markers from linkage maps were physically mapped.

Invited speaker, Dr. Sue Naylor, Department of Cellular and Structural Biology, University of Texas Health Center, in San Antonio, presented a very interesting overview of the human chromosome 3 project. This project has over 50 scientists working on chromosome 3 and the progress in both gene mapping and identification of disease genes was exciting.

The final speaker was Dr. T. C. Hsu, Department of Cell Biology, M.D. Anderson Hospital and Tumor Institute, Houston, Texas. Dr. Hsu gave an insightful and humorous talk on "A historical overview of mammalian cytogenetics." He overviewed the amazing progress in cytogenetics over the last few decades.

In addition to the invited speakers, there were 28 short presentations and 19 abstracts covering many species and a great deal of physical and genetic mapping including results in the pigs, which will be added to PiGBASE. Dr. Jim Womack and Ms. Penny Riggs and their colleagues did an excellent job. Next year's meeting will be in Zaragoza, Spain June 26-28, 1996.

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Florida was the site for the American Society of Animal Science meetings that featured many interesting papers in several sections. One section was devoted to " Molecular Markers and Quantitative Trait Loci" and included papers on pig quantitative trait loci.

Results were discussed from University of Wisconsin and Oklahoma State University on GH and IGF1 relationships to growth and carcass traits, Brigham Young University on RAPD markers and growth and carcass traits and PIC presented continued ESR results for litter size.

Papers on QTL in mice and cattle and new statistical approaches were also presented orally and at the poster session. Abstracts are published in J. Anim. Sci. 73 (supplement 1). Appropriate abstacts will be added to the database shortly.

In addition, the Contemporary Issues Symposium discussed implications related to genetic engineering and patenting. Dr. James Lauderdale from Upjohn spoke on genetically modified animals and offered suggestions on working with the public and in obtaining public support for research in the animal sciences. Dr. Beth Lautner from NPPC discussed use of antibiotics in the livestock industry. Dr. Thomas Fretz, Dean of the College of Agiculture at the University of Maryland, gave an excellent talk on the role of patenting and highlighted how industry and universities should work together.

An additional 25 pairs will be produced this month and be available. This brings the total to 299 pairs of primers that have been shipped to gene mappers and QTL researchers in over 26 labs in 10 countries.

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Ideas from pig gene mappers are needed as the new fiscal year is approaching. In May, we helped organize and host a conference on automated genotyping and have been visiting with those members of PiGMaP currently using automated genotyping in order to further develop this technology in the U.S.

We are now compiling a list of microsatellite primers that could be used in automated genotyping projects. Once a list is complete, we will consider making fluorescent markers for some or all of these primers. Other ideas of services that can be provided as parts of the coordination effort are under consideration. Your thoughts on these matters would be appreciated.

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Information on inherited disorders in pigs can now be accessed through the internet. A catalogue of inherited disorders in pigs is now available on our pig genome WWW page and on the Internet.

It is part of Mendelian Inheritance in Animals (MIA) which is a catalogue of genes and phenes (familial traits) in animal species other than mouse, rat and primates. The MIA is modelled on, and is complementary to, Dr. Victor McKusick's Mendelian Inheritance in Man (MIM). The online version of MIA is called OMIA. It can be searched in exactly the same way as OMIM, which is the online version of MIM. The OMIA is presently available on our pig gene mapping home page and from the home page of the Australian National Genomic Information Service (ANGIS) at the University of Sydney [http://morgan.angis.su.oz.au].

OMIA is also accessible in ACeDB format from the Agricultural Genomic Information Server (AGIS) of the USDA's National Agricultural Library [http://probe.nalusda.gov]. In both formats, if there appears to be from the pig a human homologue of an animal phene, the relevant MIM number provides a direct link to the relevant entry in OMIM.

The present version of OMIA contains bibliographic details for 7219 publications on 1111 phenes across 21 species, including 1375 publications on 222 phenes in pigs. The catalogue is updated continually but is incomplete in many ways, and the editor, Frank Nicholas, would be grateful for suggestions for improvements (including suggestions for references that should be included). He can be reached at [frankn@doolittle.vetsci.su.oz.au] (kindly provided by Frank Nicholas).

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Don't forget the NRSP-8 meetings and the pig gene mapping NC-210 meeting. The annual meeting of the National Genome Research Program Technical committees (NSRP8) will be held October 2527, 1995 at the University of Maryland Continuing Education Center (301- 9857303). Rooms are $74 for a single and $89 for a double. Organizers include Dr. Joan Lunney (housing and registration), Dr. Brian Kirkpatrick (keynote speakers) and Dr. Sue Lamont (workshop speakers). Rooms are reserved under the title RUSDA Swine Poultry Cattle Genome Meetings. Tentative schedules for the meetings include:
Wednesday, Oct. 25	NC168 Poultry meetings and arrival 
			of other attendees.
Thursday, Oct. 26	Individual species meetings during 
			day, social and executive committee 
			meeting in the evening.
Friday, Oct. 27		Keynote speakers (from NIH and National 
			Cancer Institute), 
			business meeting, funding workshops 
			(ending by 3 pm.).

Agenda items will include a proposal to start an Animal Genome meeting in conjunction with the Plant Genome meetings (usually held in San Diego) in early spring of 1997, updates on database progress, and plans for continuation of coordinated animal genome work after current project ends in 1998. Please contact your species chair or email me at mfrothsc@iastate.edu if you have questions on attending.

Contributions to Pig Genome Update 15 including short meeting announcements are always welcome. Please send by the 15th of October.

                    Max Rothschild
                    U.S. Pig Genome Coordinator
                    2255 Kildee Hall, Department of Animal Science
                    Iowa State University
                    Ames, Iowa 50011
                    Phone: 515-294-6202, Fax: 515-294-2401

cc: Dick Frahm, CSREES and Roger Gerrits, ARS
Paid for by funds from the NRSP-8
USDA/CSREES sponsored
Pig Genome Coordination Program
Mailing list: angenmap@db.genome.iastate.edu

© US Pig Genome Coordination Program