From listmaster animalgenome.org Wed Jan 15 12:34:33 2020
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From: Elizabeth Ross <e.rossuq.edu.au>
Subject: Re: DNA extraction from cattle hair for WGS
Postmaster: submission approved by list moderator
To: Members of AnGenMap <angenmapanimalgenome.org>
Date: Wed, 15 Jan 2020 12:34:33 -0600
Hi Laura, we have never noticed a problem with degredation. I know VicDPI
were using it for long term dna storage and it is sold as "archive quality".
We do get shorter DNA from hair than semen but there doesn't appear to be
any further shearing once the DNA is extracted.
For long read sequencing we have added a few tweaks to the protocol. If you
are doing ont or pacbio we are happy to share info on that, just let me know
and I can email it though when I am back in Aus (currently at PAG).
Cheers.
On Jan 16, 2020 3:30 AM, Laura Iacolina <lauraiacolinagmail.com> wrote:
Liz, is it stable? We sometimes have problem with Qiagen kits (never tried
the Gentra though) and sources as hair and bone. Results are ok, but we
need to perform the analysis within a short time 7-10 days otherwise it
degrades and is no longer suitable.
Thanks,
Laura
--
PhD Laura Iacolina
Mammalian Biology subject editor
https://www.researchgate.net/.../Laura_Iacolina/info
https://lauraiacolina.wordpress.com/
Il giorno mer 15 gen 2020 alle ore 18:16 Elizabeth Ross <mailto:e.rossuq.edu.au> ha scritto:
We use Gentra Puregene Tissue Kit from qiagen on tail hair and use the DNA
for nanopore sequencing. It gives great purity and excellent length if you
are gentle with it.
Cheers
Liz
On Jan 16, 2020 1:32 AM, Rick Tearle <mailto:rick.tearleadelaide.edu.au> wrote:
Does anyone have a good method for extracting DNA from cattle hair?
We need it for whole genome sequencing so has to be high MW.
Methods that work for genotyping do not appear to work for WGS,
Dr Rick Tearle
Senior Bioinformatician
Davies Research Centre
University of Adelaide Roseworthy Campus
rick.tearleadelaide.edu.au
+61 432 07 58 07
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