Cattle QTL Database

Trait Information

Trait name:

Antibody-mediated immune response

Vertebrate Trait Ontology:

Immune response trait

Reported name:

E. coli O157 Vaccine Response

Product Trait Ontology:

n/a

Symbol:

ABIR

Clinical Measurement Ontology:

n/a

QTL Map Information

Chromosome:

QTL Peak Location:

91.65 (cM)

QTL Span:

91.65-91.65 (cM)
95.7-95.7 (Mbp)

Upper, "Suggestive":

n/a

Upper, "Significant":

n/a

Peak:

Lower, "Significant":

n/a

Lower, "Suggestive":

n/a

Marker type:

Analysis type:

Association

Model tested:

Mendelian

Test base:

Genome-wise

Threshold significance level:

Significant

P_values

7.8

Dominance effect:

0.19

Additive effect:

0.23

Associated Gene:

n/a

Cis/Trans acting type:

Links: Edit | Map view

Extended information:

(none)

QTL Experiment in Brief

Animals:

Calves (n=483) from the USDA-Meat Animal Research Center Germplasm Evaluation Program were sampled. Calves were crossbred and germplasm from 16 beef cattle breeds and composites are represented including Angus, Beefmaster, Brahman, Brangus, Braunvieh, Charolais, Chiangus, Gelbvieh, Hereford, Limousin, Maine Anjou, Red Angus, Salers, Santa Gertrudis, Shorthorn, and Simmental. Calves were born between March and June 2012 and averaged 127 days of age at time of initial sampling. Dams of calves averaged 6.8 years of age. Calves were raised at four locations separated by at least two miles on 50 square miles of federal property at the USDA Meat Animal Research Center (about 4 miles west of Clay Center, Nebraska, USA). Animal breed (IDs) involved: 537294547486089102135168182186189192

Breeds associated:

n/a

Design:

QTL for humoral response to an Escherichia coli O157 vaccine were mapped in a genome-wide association study (GWAS). Calves were vaccinated (d 0) and serum was collected at two time points: d 0 and d 21. Our humoral vaccine response phenotype was the following: E. coli O157 specific antibodies at d 21 minus d 0. Number of progeny per sire varied between 1 and 37 with an average of 5.03 progeny per sire. Ninety-six sires were used to produce calves: 288 calves were in 16 half-sibships comprised of 10-37 progeny per sire, 154 calves were in 39 half-sibships comprised of 2-9 progeny per sire, and 41 calves were in single sire families. Calves were genotyped with the Bovine GeneSeek GGP-HD SNP Beadchip by Neogen Corporation (Lincoln, NE, USA).

Analysis:

A linear model was used to estimate SNP effects on vaccine response, where our dependent variable was the phenotype (vaccine response) and independent variables were fixed effects of SNP genotype, birth location, and gender, covariates calf age, pedigree-derived expected heterozygosity, and breed effects (using breed percentages), and random additive animal effects estimated with a genomic relationship matrix. Effect of SNPs on vaccine response were estimated for each SNP individually. A false-discovery rate P-value = 0.05 was used to identify SNPs associated with vaccine response.

Software:

R software

Notes:

Links:

Edit

Reference

Authors:

Kara B. Marley, Larry A. Kuehn, John W. Keele, Benjamin W. Wileman, and Michael G. Gonda

Affiliation:

South Dakota State University

Title:

Genetic variation in humoral response to an Escherichia coli O157:H7 vaccine in beef cattle

Journal:

PLoS One, 2018, 13(5)

Links:

PubMed | Abstract | List all data | Edit

Cite this Data:

Animal QTLdb: QTL140312 was published in 2018, and was curated into QTLdb on 2017-11-13. DBxREF link to this data: https://www.animalgenome.org/QTLdb/q?id=QTL_ID:140312

QTL 140312 Description: