From leif.andersson@bmc.uu.se Fri May 19 04:33:38 2000 Message-Id: <3.0.6.32.20000519114353.00a05260@hermes.bmc.uu.se> Date: Fri, 19 May 2000 11:43:53 +0200 From: Leif Andersson Subject: ost-doctoral positions in Uppsala, Sweden To: Multiple Recipients of Functional genome analysis using divergent intercrosses in farm animals ------------------------------------------------------------------------ We are currently developing three large resource pedigrees for mapping phenotypic trait loci in farm animals. On is a cross between Hampshire and Landrace pigs, two breeds both selected for meat production but showing striking phenotypic differences for a variety of traits. The phenotypic characterization includes growth, fat deposition, muscle characteristics and fertility. The second cross involves the Red Jungle Fowl and a domestic egg-laying chicken (White leghorn). These two populations show dramatic differences for a number of traits, including fertility (egg production!), behavior, and growth. The third intercross concerns two selection lines in chicken divergently selected for growth for more than 40 generations. The High line animals are hyperphagic and develop obesity while the Low line tend to be anorectic and show very poor growth. This intercross will provide a unique resource for mapping genes controlling growth, appetite, muscle development and fat deposition. The strategy will be to carry out genome scans with microsatellite markers followed by comparative positional cloning or positional cloning to identify causative genes. We also have the ambition to combine genetic mapping with expression analysis using cDNA arrays. At least one two-year post-doctoral position is immediately available in this project. The succesful applicant need a PhD from relevant area and considerable experience of microsatellite analysis using a DNA sequencer. Positional cloning of X-linked genes for sex determination in XY sex reversed wood lemmings ----------------------------------------------------------------------------- The wood lemming is a unique model for studying the role of X-linked genes in mammalian sex determination due to the presence of a mutated form of X chromosome, X* (Fredga et al. 1976, Nature 261:225.). X* converts Y-carrying embryos into females. X*Y sex reversed females only transmit X* and only give birth to daughters. Opposite to sex reversed mice and men, the X*Y females are fully fertile. Cytogenetic analyses have demonstrated that the X* chromosome involves an inversion and a deletion of a few mega basepairs. We assume that one or more X-linked genes needed for normal male development are deleted or interrupted on the X* chromosome. The aim of this project is to clone and characterise gene(s) responsible for XY sex reversal in the wood lemming. A post-doctoral position for two years in this project is immediately available. The successful applicant need a PhD from a relevant area and a strong background in genome analysis. APPLICATIONS: Submit a letter of interest by email including CV, experience, qualifications, list of publications, and names, addresses and telephone numbers of two references to: Leif.Andersson@bmc.uu.se. Please indicate which project you are most interested in. ----------------------------------------------------------------------------- Leif Andersson Phone:+46-18 4714904 Department of Animal Breeding and Genetics Fax: +46-18 504461 Swedish University of Agricultural Sciences E-mail:Leif.Andersson@bmc.uu.se Uppsala Biomedical Center Box 597 S-751 24 Uppsala Sweden ----------------------------------------------------------------------------- >